Micro-Preparative HPLC

Higgins Analytical Cartridge Columns are

helping researchers understand the

immune response of living cells. An investigation

to understand the genetic control

of the immune response systems in

organisms involves the extraction, isolation,

and identification of more than

10,000 peptides. These distinct peptides

are bound to a major histocompatibility

complex (MHC) molecule on tumor

cells.

Extraction of approximately 5x10¹⁰ cells

yield several nanomoles of a very complex

peptide mixture. Several rounds of

RP-HPLC with different organic modifiers

on a 40x2.1mm PEEK cartridge column

(HAISIL 300 C18 5μ m, P/N: HK-0421-

W185) are performed to fractionate the

mixture into individual components.

The chromatograms on the left illustrate

how this technique is being used to narrow

the search for the one peptide that

marks a particular tumor cell as being

diseased enabling it to be recognized by

the immune cells.

For Biomolecules

Phases C18

Particle Sizes 5 and

Pore Size 300Å

Pore Volume 0.7mL/gm

Surface Area 80m