NUCLEOSIL® standard silica phases for HPLCFeatures![](http://www.mn-net.com/Portals/4/images/Redakteure_Chroma/hexagon.gif) | NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure
![](http://www.mn-net.com/Portals/4/images/Redakteure_Chroma/Listenzeichen_mit.jpg) Wide acceptance as routine packings for very different fields of chromatography ![](http://www.mn-net.com/Portals/4/images/Redakteure_Chroma/Listenzeichen_mit.jpg) One of the first spherical silicas used in HPLC ![](http://www.mn-net.com/Portals/4/images/Redakteure_Chroma/Listenzeichen_mit.jpg) Developed in the early seventies, it became a world-renowned HPLC packing ![](http://www.mn-net.com/Portals/4/images/Redakteure_Chroma/Listenzeichen_mit.jpg) Still found in many analytical and preparative applications ![](http://www.mn-net.com/Portals/4/images/Redakteure_Chroma/Listenzeichen_mit.jpg) An absolutely reliable choice in HPLC ![](http://www.mn-net.com/Portals/4/images/Redakteure_Chroma/Listenzeichen_mit.jpg) The largest variety of modified HPLC silicas available on the market | ![](http://www.mn-net.com/Portals/4/images/Redakteure_Chroma/HPLC/Rem-Nucleosil.gif) | Summary of available NUCLEOSIL® phasesPhase | Modification | Formula | RP phases | NUCLEOSIL® C18 | medium density octadecyl, endcapped | -(CH2)17-CH3 | NUCLEOSIL® C18 AB | crosslinked octadecyl, endcapped, high steric selectivity | -(CH2)17-CH3 | NUCLEOSIL® C18 HD | high density octadecyl, endcapped | -(CH2)17-CH3 | NUCLEOSIL® C18 Nautilus | hydrophilic octadecyl, embedded polar group, endcapped | -(CH2)17-CH3 | NUCLEOSIL® Protect I | special RP phase with protective polar group, endcapped | | NUCLEOSIL® C8 ec | medium density octyl, endcapped | -(CH2)7-CH3 | NUCLEOSIL® C8 | standard octyl, not endcapped | -(CH2)7-CH3 | NUCLEOSIL® C8 HD | high density octyl, endcapped | -(CH2)7-CH3 | NUCLEOSIL® C4 | medium density butyl, endcapped | -(CH2)3-CH3 | NUCLEOSIL® C2 | dimethyl | -(CH3)2 | NUCLEOSIL® C6H5 | standard phenyl, not endcapped | -(CH2)3-C6H5 | Polar phases | NUCLEOSIL® CN | cyanopropyl (nitrile) | -(CH2)3-CN | NUCLEOSIL® OH | diol (dihydroxypropyl) | -(CH2)3-O-CH2-CH(OH)-CH2OH | NUCLEOSIL® NH2 | aminopropyl | -(CH2)3-NH2 | NUCLEOSIL® N(CH3)2 | dimethylamino | -(CH2)3-N(CH3)2 | NUCLEOSIL® SA | benzenesulfonic acid, strongly acidic cation exchanger (SCX) | -(CH2)3-C6H4-SO3Na | NUCLEOSIL® SB | quaternary ammonium groups, strongly basic anion exchanger (SAX) | -(CH2)3-C6H4-CH2-N+(CH3)3Cl– | | | | NUCLEOSIL® SiOH | unmodified | (SiO2)n | Technical characteristics![](http://www.mn-net.com/Portals/4/images/Redakteure_Chroma/hexagon.gif) | Spherical silica, pH stability 2–8 Available pore sizes 50, 100, 120, 300, 500, 1000 and 4000 Å Available particle sizes 3 µm, 5 µm, 7 µm and 10 µm | | Physical properties of unmodified NUCLEOSIL® materials | | Phase | Pore size | Pore volume | Surface (BET) | Density | Pressure stability* | | NUCLEOSIL 50 | 50 Å | 0.8 mL/g | 420 m2/g | 0.45 g/mL | 500 bar | | NUCLEOSIL 100 | 100 Å | 1 mL/g | 350 m2/g | 0.36 g/mL | 500 bar | | NUCLEOSIL 120 | 120 Å | 0.65 mL/g | 200 m2/g | 0.55 g/mL | 500 bar | | NUCLEOSIL 300 | 300 Å | 0.8 mL/g | 100 m2/g | 0.45 g/mL | 400 bar | | NUCLEOSIL 500 | 500 Å | 0.8 mL/g | 35 m2/g | 0.45 g/mL | 400 bar | | NUCLEOSIL 1000 | 1000 Å | 0.8 mL/g | 25 m2/g | 0.45 g/mL | 300 bar | | NUCLEOSIL 4000 | 4000 Å | 0.7 mL/g | 10 m2/g | 0.48 g/mL | 300 bar | | * Maximum packing pressure; for maximum working pressure of packed columns please refer to the instruction leaflets. | |
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