NUCLEOSIL® standard silica phases for HPLCFeatures | NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure
 Wide acceptance as routine packings for very different fields of chromatography One of the first spherical silicas used in HPLC Developed in the early seventies, it became a world-renowned HPLC packing Still found in many analytical and preparative applications An absolutely reliable choice in HPLC The largest variety of modified HPLC silicas available on the market |  | Summary of available NUCLEOSIL® phases| Phase | Modification | Formula | | RP phases | | NUCLEOSIL® C18 | medium density octadecyl, endcapped | -(CH2)17-CH3 | | NUCLEOSIL® C18 AB | crosslinked octadecyl, endcapped, high steric selectivity | -(CH2)17-CH3 | | NUCLEOSIL® C18 HD | high density octadecyl, endcapped | -(CH2)17-CH3 | | NUCLEOSIL® C18 Nautilus | hydrophilic octadecyl, embedded polar group, endcapped | -(CH2)17-CH3 | | NUCLEOSIL® Protect I | special RP phase with protective polar group, endcapped | | | NUCLEOSIL® C8 ec | medium density octyl, endcapped | -(CH2)7-CH3 | | NUCLEOSIL® C8 | standard octyl, not endcapped | -(CH2)7-CH3 | | NUCLEOSIL® C8 HD | high density octyl, endcapped | -(CH2)7-CH3 | | NUCLEOSIL® C4 | medium density butyl, endcapped | -(CH2)3-CH3 | | NUCLEOSIL® C2 | dimethyl | -(CH3)2 | | NUCLEOSIL® C6H5 | standard phenyl, not endcapped | -(CH2)3-C6H5 | | Polar phases | | NUCLEOSIL® CN | cyanopropyl (nitrile) | -(CH2)3-CN | | NUCLEOSIL® OH | diol (dihydroxypropyl) | -(CH2)3-O-CH2-CH(OH)-CH2OH | | NUCLEOSIL® NH2 | aminopropyl | -(CH2)3-NH2 | | NUCLEOSIL® N(CH3)2 | dimethylamino | -(CH2)3-N(CH3)2 | | NUCLEOSIL® SA | benzenesulfonic acid, strongly acidic cation exchanger (SCX) | -(CH2)3-C6H4-SO3Na | | NUCLEOSIL® SB | quaternary ammonium groups, strongly basic anion exchanger (SAX) | -(CH2)3-C6H4-CH2-N+(CH3)3Cl– | | | | | | NUCLEOSIL® SiOH | unmodified | (SiO2)n | Technical characteristics | Spherical silica, pH stability 2–8
Available pore sizes 50, 100, 120, 300, 500, 1000 and 4000 Å
Available particle sizes 3 µm, 5 µm, 7 µm and 10 µm | | | Physical properties of unmodified NUCLEOSIL® materials | | | Phase | Pore size | Pore volume | Surface (BET) | Density | Pressure stability* | | | NUCLEOSIL 50 | 50 Å | 0.8 mL/g | 420 m2/g | 0.45 g/mL | 500 bar | | | NUCLEOSIL 100 | 100 Å | 1 mL/g | 350 m2/g | 0.36 g/mL | 500 bar | | | NUCLEOSIL 120 | 120 Å | 0.65 mL/g | 200 m2/g | 0.55 g/mL | 500 bar | | | NUCLEOSIL 300 | 300 Å | 0.8 mL/g | 100 m2/g | 0.45 g/mL | 400 bar | | | NUCLEOSIL 500 | 500 Å | 0.8 mL/g | 35 m2/g | 0.45 g/mL | 400 bar | | | NUCLEOSIL 1000 | 1000 Å | 0.8 mL/g | 25 m2/g | 0.45 g/mL | 300 bar | | | NUCLEOSIL 4000 | 4000 Å | 0.7 mL/g | 10 m2/g | 0.48 g/mL | 300 bar | | | * Maximum packing pressure; for maximum working pressure of packed columns please refer to the instruction leaflets. | |
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