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Proto 200 C18 Proto 200 C4

Applications

300Å wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120Å) typically used for small molecule analysis. 300Å sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200 presents a powerful alternative for the analysis of peptides of ~19kD and less.

Phosphopeptide Purity Analysis

Peptide HPLC column performance, using the same gradient conditions, was evaluated on the PROTO™ 200 C18. Against the industry standard, Grace/Vydac® 218TP C18, and their newer peptide chemistry, Everest® C18, the data show that the PROTO™ 200 C18 column has superior resolution to the 218TP chemistry and better resolution, especially for the more hydrophobic peptides, than the Everest® C18. In addition, the PROTO 200 columns can be run without TFA, which allows better detection when utilizing LC-MS. Previously, the Everest chemistry had been shown by Grace/Vydac to be superior to all other wide pore columns, especially for the more hydrophobic peptides. However, the ready availability of the PROTO™ 200 peptide columns, in either C4 or C18 chemistries, and in capillary through preparative sizes, now allows researchers their first opportunity to save both time and money on a wider variety of applications, compared to the Grace/Vydac columns, and by extension to all other peptide columns.

PROTO200 More Selectivity and Resolution for Peptides

Phases C4 and C18

Particle Sizes 4.5 and 10μm

Pore Size 200Å

Pore Volume 1.1mL/gm

Surface Area 200m

2/gm

%Carbon (w/w) C4. = 4.5%, C18= 14%

Phase type Monofunctional & fully endcapped

Silica Class Type B

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Proto 200 C4 5um 1.0 x 10.0mm Piccolo Column Proto 200 C18 5um 1.0 x 10.0mm Piccolo Column Proto 200 C4 5um 0.3 x 2.5mm Piccolo Column
RP-0101-D045 RP-0101-D185 RP-M2M3-D045
Proto 200 C18 5um 0.3 x 2.5mm Piccolo Column Proto 200 C4 5um 0.5 x 2.5mm Piccolo Column Proto 200 C18 5um 0.5 x 2.5mm Piccolo Column
RP-M2M3-D185 RP-M2M5-D045 RP-M2M5-D185
Proto 200 C4 5um 1.0 x 5.0mm Piccolo Column Proto 200 C18 5um 1.0 x 5.0mm Piccolo Column Proto 200 C4 5um 2.1 x 10mm Sprite Column
RP-M501-D045 RP-M501-D185 RR-0121-D045
Proto 200 C18 5um 2.1 x 10mm Sprite Column Proto 200 C4 5um 2.1 x 20mm Sprite Column Proto 200 C18 5um 2.1 x 20mm Sprite Column
RR-0121-D185 RR-0221-D045 RR-0221-D185
Proto 200 C4 5um 2.1 x 40mm Sprite Column Proto 200 C18 5um 2.1 x 40mm Sprite Column Proto 200 C4 5um 1.0 x 30.0mm Piccolo Column
RR-0421-D045 RR-0421-D185 RP-0301-D045
Proto 200 C18 5um 1.0 x 30.0mm Piccolo Column Proto 200 C18 5um 4.6 x 30mm Analytical Column Proto 200 C4 5um 0.075 x 250mm Capillary Column
RP-0301-D185 936518 RC-2507-D045
Proto 200 C18 5um 0.075 x 250mm Capillary Column Proto 200 C4 5um 0.150 x 250mm Capillary Column Proto 200 C18 5um 0.150 x 250mm Capillary Column
RC-2507-D185 RC-2515-D045 RC-2515-D185
Proto 200 C4 5um 0.8 x 30mm Microbore Column Proto 200 C4 10um 10 x 50mm Semi Prep Column Proto 200 C18 10um 10 x 50mm Semi Prep Column
RS-03M8-D185 RS-0510-D041 RS-0510-D181
Proto 200 C4 5um 0.8 x 50mm Microbore Column Proto 200 C4 5um 2.1 x 30mm Analytical Column Proto 200 C18 5um 2.1 x 30mm Analytical Column
RS-05M8-D185 RS-0321-D045 RS-0321-D185
Proto 200 C4 5um 3.0 x 30mm Analytical Column Proto 200 C18 5um 3.0 x 30mm Analytical Column Proto 200 C4 5um 4.0 x 30mm Analytical Column
RS-0330-D045 RS-0330-D185 RS-0340-D045
   
 
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