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o-Phthalaldehyde (OPA), ea. (5g/bottle)
o-Phthalaldehyde (OPA), ea. (5g/bottle)
 

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Product Number: 938032
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Description
 
Chromatographic Grade™

For Automated Post-column Derivatization of Primary Amines

Primary amines form highly fluorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptan under basic conditions. The products of this reaction, 1-alkyl-2alkylthio-substituted isoindoles, exhibit optimal excitation at 330 nm and maximal emission at 465 nm. Pickering's OPA is specially prepared to meet the demanding requirements of high-sensitivity pre- and post-column HPLC derivatization methods. Our repurification process actually begins with commercially available 99% material. The entire process is controlled to eliminate trace interfering contamination. Lot quality is verified by post-column HPLC using a high-sensitivity fluorescence detector.


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