 |
 |
 |
|
The Alltech® Anion/R Column is packed with a poly (styrenedivinylbenzene)
based anion exchanger with trimethyl
ammonium functional groups. The packing material is 10?m in
size and stable in the pH range of 2-12.
About the Column
The Alltech® Anion/R Column separates inorganic anions by ion
chromatography (IC) methods. The column can be used for
both single-column and suppressor-based IC applications.
Figure 1 shows the separation of fluoride, chloride, nitrite,
bromide, nitrate, phosphate and sulfate using p-hydroxybenzoic
acid mobile phase with conductivity detection in the single
column IC mode. The separation of seven common anions plus
sulfite is shown in Figure 2 using a carbonate/bicarbonate
mobile phase and suppressed conductivity detection. This
column is also suitable for the separation of weak acid anions
such as fluoride, borate, cyanide, silicate and sulfide. Figure 3
shows the separation of weak acid anions using sodium
hydroxide mobile phase and indirect conductivity detection.
Care and Use
The Alltech® Anion/R Columns are individually tested using
p-hydroxybenzoic acid mobile phase. A test chromatogram is
enclosed with each column. Duplicate the test chromatogram
on your instrument as soon as you receive the column. Contact
Grace or your Grace representative if you have any problems at
this time.
Ion exchange columns, like all liquid chromatographic columns
can be damaged after extended use. This problem is visible as
a loss in efficiency and column performance. A column can be
damaged by using incompatible solvents, exceeding column
pH limitations, or not filtering the sample and the mobile phase.
Best Insurance for a Long Column Life
Filter all samples and mobile phases through 0.5?m (or smaller)
filters before analyses. Use only IC grade water for preparation
of mobile phases and standards.
• Use pure reagents for mobile phase preparation.
• Pretreat samples with Alltech® Maxi-Clean™ SPE devices to
remove interferences or contaminants.
• Use a guard column when injecting real world samples.
• The guard column protects the analytical column from
contaminants. The Alltech® All-Guard™ GA-1 or Anion/R
guard cartridges are recommended for use with the Alltech®
Anion/R Columns.
In some cases, a contaminated column can be rejuvenated by
the following regeneration procedure. In other cases, the
column must be replaced.
Regeneration Procedure for the Alltec
Anion/R Column
Pump the following solvents through the column sequentially at
1mL/min:
1. 100mL 50mM disodium EDTA (adjusted to pH 10.0
withsodium hydroxide)
2. 100mL deionized water
3. 100mL 50mM sulfuric acid in 50% acetonitrile:water
4. 100mL deionized water
After the regeneration, equilibrate the column with mobile phase
and inject a test sample. If performance is still poor, replace the
column.
Storage Solvent
The Alltech® Anion/R Column can be stored in the mobile
phase, if it is stored for a short period of time. For long time
storage, store the column in 100% Methanol.
|
|
|
 |
|
 |
|
